Functionalized Nano-adsorbent for Affinity Separation of Proteins

Affinity Based Nano-Magnetic Particles for Purification of Recombinant Proteins in Form of Inclusion Body

Background: Protein purification is the most complicated issue in the downstream processes of recombinant protein production; therefore, improved selective purification methods are important. Affinity-based protein purification method using His-tag and Ni-NTA resins is one of the most common strategies. MNPs can be used as a beneficial alternative for Ni-NTA resins. However, there is no data on...

Advances in affinity ligand-functionalized nanomaterials for biomagnetic separation.

The downstream processing of proteins remains the most significant cost in protein production, and is largely attributed to rigorous chromatographic purification protocols, where the stringency of purity for biopharmaceutical products sometimes exceeds 99%. With an ever burgeoning biotechnology market, there is a constant demand for alternative purification methodologies, to ameliorate the depe...

Inertial Microfluidics for Multiplexed Affinity Separation of Proteins and Cells

We present an inertial microfluidic platform for rapid, multiplexed affinity purification of proteins and cells from complex mixtures. Beads of different sizes, coated with different bait molecules bind specific proteins or cells in a single reaction step and the bound targets are then size-sorted by flowing in a spiral microchannel. We use this scheme to simultaneously purify antibodies specif...

Affinity-Based Methods for the Separation of Parasite Proteins

Affinity chromatography has been demonstrated to be a powerful tool for the isolation and purification of parasite proteins and has potential applications for diagnosis and therapy. Many studies have focused on parasite proteins that modulate host cell defense, as gp63, a glycoprotein from Leishmania spp., that is involved in the cleavage of the complement factor C3b to iC3b, which promotes adh...

Fabrication and Evaluation of Functionalized Nano-titanium Dioxide (F-NanoTiO2)/ polysulfone (PSf) Nanocomposite Membranes for Gas Separation